|Recurring or debuting plot points in this episode:
- Googly eyes: Jeff and Britta's relationship has progressed to the point where they eat breakfast together. He also actually reflected on her criticisms of him and changed her information in his cellphone contact list from a broad description to her actual name.
- Middle Eastern Magic 8 Ball: As Abed warns about the defective condoms on the PA, a couple in the background hears him and throws away the condom they are holding. This is possibly the same couple Abed helps to deliver a baby nine months later in the Season Two episode "The Psychology of Letting Go".
|Recurring or debuting characters in this episode:
|List of Running gags
|Recurring or debuting running gags in this episode:
- Attention students!: Abed uses the public address system to warn everybody about the defective condoms.
- Biggest laugh of the night: Pierce spills Doreen's "STD-ini" on the floor while sitting down.
- My other half: Troy says that Abed is difficult to guard in basketball because his "eyes are too gentle and mysterious".
- Nice outfit: Dean Pelton wears a STD Fair T-shirt without changing out of his dress shirt and tie.
- WWBJD: Shirley tells Annie to be proud of being a virgin.
|List of Pop culture references
|References to popular culture in this episode:
- When Troy wants to armwrestle, Abed channels Sylvester Stallone in Over the Top, mimicking the backwards hat and trademark finger roll of Stallone's character.
- Both Officer Cackowski and Dean Pelton use the term "reverse Porky's" when the trio of Annie, Britta and Shirley attempt to break into the Dean's Office.
- Doreen comments on Sabrina's youth: "She thinks that Monty Python is the evil snake from Harry Potter."
- Name that tune:
- "Whistle for the Choir" by The Fratellis is heard during the conversation between Jeff and Pierce towards the end of the episode, and when Jeff changes his contact "Hot Blonde Spanish Class" to "Britta" on his phone.
- Annie had relations with her high school boyfriend while listening to Madonna's Erotica.
- Shout out:
- Shirley mentions Harvey Keitel in an unsavory light.
- Jeff casually tells Sabrina that he listens to Jay-Z after the Dean tells him she likes rap music; whether Jeff actually listens to Jay-Z is unresolved.
|List of Meta references
|Meta references in this episode:
- Annie's account of intercourse with her high-school boyfriend is strikingly similar to an incident from Alison Brie's college days.
- Abed being in better shape than Troy at the end of their sprint is one to Danny Pudi being a marathon runner.
- Some of the games that can be seen at the STD Fair include "Wheel of Remorse", "Whack an STD", "Toss STDs!", "Flip it Good!" and "What Can Really Happen?".
- Abed is wearing a hat with "STD-fense" on it.
- “Sabrina is my new secretary.”— Dean Pelton
- “'Secretary' is a little degrading to women. I help the Dean do office-y things.”— Sabrina
- “Oh, gender saved.”— Britta
- “You know the toilets in the women's bathrooms don't have seats, right?”— Britta
- “Because they keep getting stolen. Sabrina, take a note: I want hidden cameras in every stall.”— Dean Pelton
- “Problem solved.”— Britta
- “This is a lot of outgoing calls: Car Wash Redhead, Tube Top R.E.M. Concert, Juror #6--that sounds above board--at least you have Mommy in here.”— Britta
- “That's not my mom.”— Jeff
- “Dude, not cool.”— Britta
- “I am not a unicorn. I had relations with my high school boyfriend. We did it to Madonna's Erotica on the floor of his walk-in closet, but he wouldn't let me look at 'it'. He cried after. And during. He's gay now.”— Annie
- “I think he was gay then.”— Britta
- “You know what? I don't want to express myself. I don't wanna sit in a room full of people and say the P-word. I like being repressed. I am totally comfortable being uncomfortable with my sexuality. And maybe, just maybe, if everyone were a little more like me, we wouldn't have to have an STD fair.”— Annie Edison